T1952

Trichostatin A, Ready Made Solution

5 mM in DMSO, from Streptomyces sp.

• Synonym(s): TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
• Empirical Formula (Hill Notation): C₁₇H₂₂N₂O₃
• CAS Number: 58880-19-6
• Molecular Weight: 302.37
• NACRES: NA.32

Properties

• biological source: Streptomyces sp.
• Quality Level: 200
• Assay: ≥98% (HPLC)
• form: DMSO solution
• concentration: 5 mM in DMSO
• technique(s): cell culture | mammalian: suitable
• antibiotic activity spectrum: fungi; neoplastics
• Mode of action: enzyme | inhibits
• shipped in: dry ice
• storage temp.: −20°C
• InChI: 1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
• InChI key: RTKIYFITIVXBLE-WKWSCTOISA-N

Description

General description

Trichostatin A is a compound of primary hydroxamic acid.

Application

Trichostatin A, ready-made solution has been used:

• as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
• to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
• to treat cells for the HDAC inhibition experiments

Biochem/physiol Actions

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves