T8787

Triton^™ X-100

Molecular Biology

• Synonym(s): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 329826885
• NACRES: NA.21

Properties

• biological source: synthetic (organic)
• grade: Molecular Biology
• description: non-ionic
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• aggregation number: 100-155
• technique(s): protein purification: suitable; protein quantification: suitable; western blot: suitable
• impurities: <1.00% water (Karl Fischer)
• pH: 9.7
• CMC: 0.2-0.9 mM (20-25°C)
• mp: 6 °C
• transition temp: cloud point 65 °C; pour point ~7 °C
• solubility: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• density: 1.06 g/mL at 25 °C (lit.)
• cation traces: Fe: <5 ppm; K: <0.05%; Na: <0.1%; heavy metals: <5 ppm
• HLB: 13.5
• application(s): hematology; histology
• foreign activity: DNase and RNase, none detected
• storage temp.: room temp
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

Application

Triton^™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas[3]
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay[4]
• To permeabilise cells during Immunofluorescent microscopic studies[5]
• As a positive control in LDH assay to determine the cell membrane integrity[6]
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[7]
• For the preparation of outer membrane protein exctract[8]
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells[9]
• In the treatment of tissue sections for Immunofluorescence labeling[10]

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

• Ideal for sensitive molecular biology research applications
• Tested for DNase and RNase
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F - closed cup
• Flash Point(C): 251 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves