As mentioned in the 'PROPERTIES' section, the volume of each vial is 1.5 mL.
W1754
Water
PCR Reagent, suitable for PCR
Synonym(s):
H2O
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1 VIAL
£32.20
5 VIALS
£133.00
£32.20
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About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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Product Name
Water, PCR Reagent
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
InChI
1S/H2O/h1H2
vapor density
<1 (vs air)
vapor pressure
3 mmHg
sterility
sterile-filtered
Quality Level
SMILES string
O
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected, RNase, none detected
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) FISH: suitable | technique(s) - | technique(s) - |
| grade PCR Reagent | grade for molecular biology | grade - | grade - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| pH 5-7 | pH 5-7 | pH - | pH - |
| bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) |
General description
PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.
Application
Suitable for polymerase chain reaction (PCR)
Water has been used:
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR[1]
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA[2]
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA[3]
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)[3]
Other Notes
Easily compare specifications for Water products with the Water specification table.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Protocols
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
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