sufficient for 10 reactions
10 reactions sufficient for 10 amplifications
10 reactions sufficient for 10 extractions
sufficient for 100 reactions
100 reactions sufficient for 100 amplifications
100 reactions sufficient for 100 extractions
sufficient for 1000 reactions
1000 reactions sufficient for 1000 amplifications
1000 reactions sufficient for 1000 extractions
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10 - Combustible liquids
The DNA extract generated using the Extract-N-Amp kit is very crude, and it is not possible to obtain a reliable 260/280 ratio. For quantitation of pure DNA, we recommend using one of our GenElute genomic DNA kits.
DNA samples can be stored for up to 6 months at 2-8°C with the tissue sample removed.
Only R4775, the REDExtract-N-Amp PCR Reaction Mix is available separately.
The shelf life of XNAT has not been determined. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt. The REDExtract-N-Amp Tissue PCR Kit can be stored at 2-8°C for up to three weeks. For long-term storage, greater than three weeks, -20°C is recommended. Do not store in a "frost-free" freezer. We recommend to store in 10 reaction aliquots.
From XNAT Technical Bulletin, troubleshooting guide table: PCR reaction may be inhibited due to contaminants in the tissue extract. Dilute the tissue extract with a 50:50 mix of extraction and neutralization solutions. To test for inhibition, include a DNA control and/or spike a known amount of template (100-500 copies) into the PCR along with the tissue extract.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
The Extract-N-Amp Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.