Cell Migration & Invasion Assays
Cancer metastasis is the cumulative result of multiple changes in tumor cells and their microenvironment that enable cells to migrate or invade healthy tissue. Our portfolio of migration assays, invasion assays, chemotactic and haptotactic Boyden chamber assays enable researchers to simulate the conditions encountered by normal and metastatic cells in vivo.
Products
BrdU Cell Proliferation Assay
This proliferation assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells.
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric
The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with colorimetric detection.
Actin Cytoskeleton / Focal Adhesion Staining Kit
The Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & DAPI for the immunofluorescent staining of actin filaments in the cytoskeleton as well as the nucleus of the cells.
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric
The Cell Invasion Assay Kit kuses a 24 well plate with 8 um pores, which is ideal for evaluation of invasive tumor cells.
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
The QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with fluorescent detection.
Cell Comb™ Scratch Assay
Glutamine and Glutamate Determination Kit
QCM Chemotaxis Cell Migration Assay, 96-well (5 µm), fluorimetric
The QCM 5 um 96-well Migration Assay utilizes a 5 um pore size, which is appropriate for studying monocyte/macrophage migration.
QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
QCM Chemotaxis Cell Migration Assay, 24-well (5 µm), colorimetric
This 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
Neurite Outgrowth Assay Kit (3 µm)
The NS220 Neurite Outgrowth Assay Kit (3 µm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base.
QCM Chemotaxis Cell Migration Assay, 96-well (3 µm), fluorimetric
The Chemicon QCM 3 uM 96-well Migration Assay provides a quick & efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents or evaluation of integrins.
QCM Chemotaxis Cell Migration Assay, 96-well (8 µm), fluorimetric
The QCM 8 uM 96-well Migration Assay utilizes a 8 um pore size, which is appropriate for leukocyte migration.
QCM Collagen Cell Invasion Assay, 24-well (8 µm), Colorimetric
The Cell Invasion Assay Kit kuses a 24 well plate with 8 um pores, which is ideal for evaluation of invasive tumor cells.
Neurite Outgrowth Assay Kit (1 µm)
The NS225 Neurite Outgrowth Assay Kit (1 μm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base.
InnoCyte Monocyte Cell Migration Assay
QCM Endothelial Cell Invasion Assay (24 well, colorimetric)
This QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert.
ProteoExtract® Native Cytoskeleton Enrichment
The ProteoExtract Native Cytoskeleton Enrichment & Staining Kit provides cytoskeleton purification detergent buffers that retain focal adhesion & actin-associated proteins while removing soluble cytoplasmic & nuclear proteins from the cell.
Cell Migration Assays
Cell migration is fundamental to embryonic development, angiogenesis, wound healing, immune response, and inflammation. Because metastatic disease is the key factor leading to cancer mortality, understanding cell migration mechanisms is critically important in cancer studies. Many cell migration assays employ a qualitative scratch assay or the more quantitative Boyden chamber technique.
Boyden chamber methods monitor cell migration based on chemotaxis and haptotaxis. Chemotaxis describes cell migration in response to extracellular chemical signals, while haptotaxis describes cell migration towards an immobilized extracellular matrix (ECM) protein gradient.
Both chemotaxis and haptotaxis QCM™ migration assays are available in different pore sizes and multiwell formats. QCM™ migration assays do not require the cell labeling, scraping, washing, and counting that is associated with other protocols involving calcein-AM labeling of cells.
InnoCyte™ cell migration assays in multi-well format are intended for chemotaxis cell migration. This assay measures the migration of epithelial, mesenchymal, and endothelial cell types through an 8 μm pore to a feeder layer using a cell-permeable fluorescent dye.
Scratch Assay for Cell Migration
A scratch assay is used to study cell migration and wound healing. The scratch assay generates a scratch on the surface of a confluent cell monolayer to create a narrow wound-like gap. The assay then monitors the migration of neighboring cells into the gap until the gap is filled. Our Cell Comb™ scratch assay creates a high-density field of scratches to maximize wound edges, while leaving enough undamaged cells to migrate into the gap.
Invasion by HT-1080 cells is determined by 96-well Cell Invasion Assay. NIH3T3 cells were used as a non-invasive control.
Cell Invasion Assays
A tumor’s ability to degrade the ECM components of the basement membrane and associated tissue is indicative of metastatic potential. Our QCM™ Boyden chamber cell invasion assays are a high-throughput and sensitive approach for quantifying cell invasion in vitro. In this assay, cells invade a model of the basement membrane, where the membrane pores are obstructed with a layer of ECM proteins, blocking non-invasive cells.
The Chemicon® neurite outgrowth assay uses Millipore® microporous tissue culture insert technology, which permits the passage of neurite processes through 1 µm pores, a diameter that does not permit cell bodies to pass. This assay is optimized for PC12 cells.
Related Resources
- Article: Cell Migration & Invasion Assays
Study tumor metastasis via various migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay, and scratch assay.
- Article: The Endothelial Cell Transwell Migration and Invasion Assay
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
- Article: Migration and Invasion Assay Chart
These charts provide an overview of various cell migration and invasion assays offered for life science research applications.
- Article: Boyden Chamber Technique
This article explains the Boyden Chamber technique for evaluating cell migration, detailing the process, appropriate pore sizes for different cell types, and the materials used in the assay.
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