Growing plant tissue in the laboratory may include seed, meristem, callus, and bud culture, and requires specialized plant culture media. Murashige and Skoog (also called MS media, MSO, or MS0) and Gamborg’s B5 medium are two of the most essential media formulations used for culturing plants. These media contain all the micro- and macronutrients, vitamins, organic supplements, and plant growth regulators that are necessary for the growth and multiplication of plant cells, tissues, and organs in vitro. For applications that require more custom formulations, we offer a comprehensive selection of basal salt mixtures, vitamin solutions, and supplements.
Much of plant tissue culture for research and biotechnology still relies on media described more than 50 years ago by Murashige and Skoog. Their eponymous Murashige and Skoog media, or MS media, is the most widely-used plant culture medium and is available as basal salt mixtures or media containing organics.
Regardless of format, MS media is comprised of micro- and macronutrients, plus nutrients like sugar, vitamins, and growth regulators. Different MS media components can be custom-combined to create the right media formulation for each plant cell or tissue type.
Gamborg′s B5 medium is an optimized culture medium for in vitro plant cell, tissue, and organ culture. It contains a mixture of inorganic salts, vitamins, and carbohydrates. We offer Gamborg’s B5 medium with minimal organics, Gamborg’s vitamin mix, and Gamborg’s B5 basal salt mix.
Phytamax™ Orchid Multiplication Medium and Phytamax™ Orchid Maintenance Medium are used for culturing, propagating and replating the orchid stem. Phytamax™ orchid culture media are available with or without activated charcoal, and with both charcoal and banana powder.