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Protein Electrophoresis & Western Blotting

Transfer Buffers & Reagents

Protein electrophoresis and Western blotting are critical methods for protein resolution and protein detection and analysis, respectively. We offer a wide variety of standard buffers, reagents, and stains for Western blotting. Our blocking, transfer, and wash buffers are all available in a variety of concentrations and package sizes. Choose from gel casting reagents or pre-cast gels, to simplify your protein resolution. Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.  

PVDF and nitrocellulose Immobilon® transfer membranes for Western blotting applications.

Immobilon Transfer Membranes & Kits

Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.

Protein Markers

Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.

mPAGE™ color protein standards for SDS-PAGE and Western blotting applications

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mPAGE™ bis-tris gels for protein electrophoresis
Protein Electrophoresis Gels & Buffers

Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.

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Protein gel electrophoresis using the Millipore® mPAGE™ gel electrophoresis system
Electrophoresis Systems & Transfer Equipment

Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.

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Protein gel stained with Coomassie blue stain
Protein Electrophoresis Gel Stains

High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.

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Western blotting reagents for detection
Immunodetection Reagents & Substrates

High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.

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Immobilon® GO immunodetection device for Western blotting
Membrane Immunodetection Devices

Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.

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Transfer Buffers & Reagents
Transfer Buffers & Reagents

Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.

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membranes (39)

sheets (25)

substrates (20)

precast gels (19)

protein standards (16)

kits (13)

chemiluminescent (72)

colorimetric (59)

fluorometric (49)

radioactive (35)

western blot (94)

dot blot (41)

electrophoresis (39)

SDS-PAGE (36)

protein staining (13)

ELISA (5)
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Immobilon<SUP>®</SUP>-P PVDF Membrane
IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Immobilon Western Chemiluminescent HRP Substrate
WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Immobilon Forte Western HRP substrate, 100 mL
WBLUF

Immobilon Forte Western HRP substrate, 100 mL

Western blotting, dot/slot blotting

Immobilon<SUP>®</SUP>-FL PVDF Membrane
IPFL00010

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

READYBLUE<SUP>®</SUP> PROTEIN GEL STAIN
RSB

READYBLUE® PROTEIN GEL STAIN

Immobilon<SUP>®</SUP>-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll
IPVH85R

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

BLUeye Prestained Protein Ladder
94964

BLUeye Prestained Protein Ladder

room temperature stable molecular weight markers

SYPRO<SUP>™</SUP> Orange Protein Gel Stain
S5692

SYPRO Orange Protein Gel Stain

MOPS SDS running buffer powder for mPAGE<SUP>®</SUP> Bis-Tris gels
MPM0PS

MOPS SDS running buffer powder for mPAGE® Bis-Tris gels

pack of 5 ea (Each packet makes 1 liter of running buffer)

ReBlot Plus Strong Antibody Stripping Solution, 10x
2504

ReBlot Plus Strong Antibody Stripping Solution, 10x

Immobilon Crescendo Western HRP substrate, 100 mL
WBLUR

Immobilon® Crescendo Western HRP substrate

Western blotting, dot/slot blotting

Sample Buffer, Laemmli 2× Concentrate
S3401

Sample Buffer, Laemmli 2× Concentrate

mPAGE<SUP>®</SUP> Mini Gel Tank
MGT

mPAGE® Mini Gel Tank

for electrophoresis of precast gels or hand cast polyacrylamide gels

Coomassie Brilliant blue R 250 (C.I. 42660)
1.12553

Coomassie Brilliant blue R 250 (C.I. 42660)

for electrophoresis Trademark of Imperial Chemical Industries PLC

mPAGE<SUP>®</SUP> 4-12% Bis-Tris Precast Gel
MP41G15

mPAGE® 4-12% Bis-Tris Precast Gels

4-12% Bis-Tris, 15 well, 1mm

mPAGE<SUP>®</SUP> 4-12% Bis-Tris Precast Gel
MP41G12

mPAGE® 4-12% Bis-Tris Precast Gels

4-12% Bis-Tris, 12 well, 1mm

MES SDS running buffer powder for mPAGE<SUP>®</SUP> Bis-Tris gels
MPMES

MES SDS running buffer powder for mPAGE® Bis-Tris gels

pack of 5 ea (Each packet makes 1 liter of running buffer)

mPAGE<SUP>®</SUP> 4-20% Bis-Tris Precast Gel
MP42G15

mPAGE® 4-20% Bis-Tris Precast Gels

4-20% Bis-Tris, 15 well, 1mm

Immobilon<SUP>®</SUP>-PSQ PVDF Membrane
ISEQ00010

Immobilon® -PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, transfer membrane for Western blotting

SIGMA<I>FAST</I><SUP>™</SUP> 3,3′-Diaminobenzidine tablets
D4418

3,3′-Diaminobenzidine

SIGMAFAST, chromogenic, tablet


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