Protein Electrophoresis & Western Blotting

Protein electrophoresis and Western blotting are critical methods for protein resolution and protein detection and analysis, respectively. We offer a wide variety of standard buffers, reagents, and stains for Western blotting. Our blocking, transfer, and wash buffers are all available in a variety of concentrations and package sizes. Choose from gel casting reagents or pre-cast gels, to simplify your protein resolution. Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.

Immobilon Transfer Membranes & Kits
Choose from a variety of Immobilon® PVDF membranes for optimal Western blotting and protein immunoblotting performance based on target protein size, application, detection method, and convenience.
Protein Markers
Pre-stained and unstained molecular weight markers for protein gel electrophoresis, SDS-PAGE, and Western blotting applications.

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Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Simplify your Western blotting workflow and save time using immunodetection devices for Western blot blocking, antibody binding, and washing.
Premixed transfer buffers for consistent, reliable protein transfer and high purity, electrophoresis-grade chemicals for making transfer buffers for Western blotting applications.
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Immobilon® -P PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Immobilon Western Chemiluminescent HRP Substrate
Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Immobilon Forte Western HRP substrate, 100 mL
Western blotting, dot/slot blotting

Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

READYBLUE® PROTEIN GEL STAIN

Immobilon®-P Membrane
1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

BLUeye Prestained Protein Ladder
room temperature stable molecular weight markers

SYPRO™ Orange Protein Gel Stain

MOPS SDS running buffer powder for mPAGE® Bis-Tris gels
pack of 5 ea (Each packet makes 1 liter of running buffer)

ReBlot Plus Strong Antibody Stripping Solution, 10x

Immobilon® Crescendo Western HRP substrate
Western blotting, dot/slot blotting

Sample Buffer, Laemmli 2× Concentrate

mPAGE® Mini Gel Tank
for electrophoresis of precast gels or hand cast polyacrylamide gels

Coomassie Brilliant blue R 250 (C.I. 42660)
for electrophoresis Trademark of Imperial Chemical Industries PLC

mPAGE® 4-12% Bis-Tris Precast Gels
4-12% Bis-Tris, 15 well, 1mm

mPAGE® 4-12% Bis-Tris Precast Gels
4-12% Bis-Tris, 12 well, 1mm

MES SDS running buffer powder for mPAGE® Bis-Tris gels
pack of 5 ea (Each packet makes 1 liter of running buffer)

mPAGE® 4-20% Bis-Tris Precast Gels
4-20% Bis-Tris, 15 well, 1mm

Immobilon® -PSQ PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.2 µm pore size, transfer membrane for Western blotting

3,3′-Diaminobenzidine
SIGMAFAST™, chromogenic, tablet
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