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72-17-3
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Data Sheet - 14781
Reserved.
Page 3 of 3
16. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy
Products, 17th Ed., APHA Inc., Washington, D.C.
17. Williams
SMC®Plate Washer Evaluation Kit
Column Mean 78 65 72 63 69 67 65 63 64 73 61 81
SD 10 9 11 12 6 10 12 10 11 11 10 5
CV 12 13 16 19 8 16 19 15 17 15
Cell Culture Poster
8
2 3
9
Media Mixes:
1) 100% A
2) 100% B
3) 100% C
4) 50% A, 50% B
5) 50% B, 50% C
6) 50% C, 50% A
Data Sheet - E4127
Orth, D. N., J. Clin. Invest. 72, 249
(1983).
21. Carpenter, G. L., J. Cell Sci., Suppl., 3, 1 (1985).
22. Thorburn, G., et al., Growth and Maturation
Factors, Vol. 3 (G. Guroff
Product Information Sheet-11449460001
234 194 118 72
https://www.sigmaaldrich.com
07
21
.1
14
53
17
30
01
18
3. Results
y Version: 18 DNA Molecular Weight Marker IX
Data Sheet - E1257
Orth, D., J. Clin. Invest., 72, 249
(1983).
21. Carpenter, G., J. Cell Sci., Suppl., 3, 1 (1985).
22. Thorburn, G. et al., Growth and Maturation Factors,
Vol. 3 (G. Guroff, ed.) Wiley and Sons
Data Sheet - I3525
polypeptide that
appeared to be involved in the regulation of the
hematopoeitic system.1-3 It shared a surprising 72%
amino acid sequence homology with the thirteenth open
reading frame of Herpesvirus saimiri
Product Information Sheet - I7375
p-Iodonitrotetrazolium Violet
I7375 Page 2 of 2
03/17/97 - CKV
references for the use of I8377 below.
For higher speed, inreased resolution and sensivity!
6
7
8
9
10
2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
700
600
500
400
300
200
Color Card 1.11117 - MQuant Ammonium Test / Ammonium-Test (Don't use printout for evaluation)
0 0.5 1 2 3 5 7 10 mg/l NH4+
0 0.4 0.8 1.6 2.3 3.9 5.4 7.8 mg/l NH4-N
B
A
3 x NH4-1
AB15
-
2
5
°C
pH
100092 FRASER Listeria Ammonium iron(III)
10-13
Merck KGaA, 64271 Darmstadt, Germany,
Tel. +49 (0)6151 72-24 40, www.merck-chemicals.com
09-112-3_PB_100092 23.11.2009 17:17 Uhr Seite 1
Product Information Sheet-ftaq-ro
min(1) 1
Denaturation
Annealing
Elongation
95
45 – 65(3)
72
30 sec
30 sec
45 sec – 3 min(4)
30 – 40(2)
Final Elongation 72 7 min 1
Cooling 4 indefinitely
Development of Cancer Cell Invasion Assays in a 96-well Format; 2001
MDAMB231 MCF7
Experimental condition
25,000 cells/30 ug/72 hr
50,000 cells/30 ug/72 hr
25,000 cells/40 ug/72 hr
50,000 cells/40 ug/72 hr
8 µ m MultiScreen TM-MIC
0
20
40
60
PCR Protocols and Guides - Simplify your gene discovery
Tm + 3 ˚C for 30 s Lowest Tm-5 ˚C for 20 s
Extend 70˚C for 2 min, 5 sec 68 ˚C for 5 min 72 ˚C for 1 min, 15 s 72 ˚C for 1 min, 15
Quality Control Range Sheet- A6602-106 and A6602-206
Control 1 101 – 210 pg/mL IL-2 Control 1 17 – 36 pg/mL
Control 2 450 – 935 pg/mL Control 2 85 – 177 pg/mL
Data Sheet - SAB4200191
Rev. Mol. Cell Biol., 9,
478-489 (2008).
3. Montagna,C., et al., Cancer Res., 63, 2179-2187
(2003).
4. Hannibal, M.C., et al., Neurology, 72, 1755-1759
(2009).
5. Gullten, T., et al.
Product Information Sheet-pwopol-ro
Annealing
Elongation
94
45 – 65(1)
72
15 sec
30 sec
45 sec – 2 min(2) + 5 sec
cycle elongation for each
successive cycle(3)
15 – 20
Final Elongation 72 up to
Product Information Sheet - 80476
acceleration with temp
66
68
70
72
74
76
78
80
82
84
86
88
90
92
94
96
98
100
Product Information Sheet - C3240
Normalization in Arabidopsis.
Plant Physiol. 139, 5-17 (2005).
2. Lovatt, A., et al. Validation of quantitative PCR
assays. BioPharm, March 2002, p. 22-32.
3. Bustin, S. A., Quantification of mRNA using
Color Card 111170 - MQuant Nitrate Test (Don't use printout for evaluation)
5.6 11 17 23 28 34 mg/l NO3-N
B
A
5 min
2 x NO3-1
AB15
-
2
5
°C
pH
4
-
1
0
A
5
Application Note - RealTime PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
20 pg
1 5 9 13 17 21 25 29 33 37 41 45
1 5 9 13 17 21 25 29 33 37 41 45 1 5 9 13 17 21 25 29 33 37 41
Application Note - Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples
1: Quality of RNA
Sample 1 Sample 2 Sample 3 Sample 4
851_GE_ANote_02_Prod_1NL.indd 3 17.03.2009 18:17:17 Uhr
4 Easy and Reliable qRT-PCR Analysis of Total RNA Isolated
Data Sheet - SAB4200372
References
1. Gomez, T.S., and Billadeau, D.D, Dev. Cell, 17,
699-711 (2009).
2. Derivery, E., et al., Dev. Cell, 17, 712-723 (2009).
3. Duleh, S.N., and Welch, M.D., Cytoskeleton, 67,
193-206
Data Sheet - SAB4200373
References
1. Gomez, T.S., and Billadeau, D.D, Dev. Cell, 17,
699-711 (2009).
2. Derivery, E., et al., Dev. Cell, 17, 712-723 (2009).
3. Duleh, S.N., and Welch, M.D., Cytoskeleton, 67,
193-206
Data Sheet - SRP5187
Lot
70-95% (densitometry)
HSD17B10
43
72
95
130
170
56
26
17
34
References
1. Yang, S.-Y. et al., Multiple functions of type 10 17-
beta-hydroxysteroid dehydrogenase. Trends
Magna ChIP2 TM Chromatin Immunoprecipitation DNA Microarray Universal Kit
minutes
Step 2: 72°C for 5 minutes
Step 3: 95°C for 2 minutes
Step 4: 95°C for 1 minute
Step 5: 60°C for 1 minute
Step 6: 72°C for 1 minute
Step 7: Go to step 4 for 22
Data Sheet - SRP5203
34
43
72
95
130
170
56
26
17
References
1. Wagner, P.D. et al., Regulation of the actin-
activated ATPase of aorta smooth muscle myosin.
J. Biol. Chem., 261(17), 7778-83 (1986
Data Sheet - D4168
Trojanowski, J.Q. et al., J. Histochem. Cytochem.,
31, 1217-1223 (1983).
3. DeJong, A.S.H. et al., Histochem. J., 17(10),
1119-1130 (1985).
4. Chu, N.M. et al., J. Histochem. Cytochem., 37
Data Sheet - M3684
Rabbit Anti-MMP-17, N-Terminal specifically binds to
MMP-17 (MT4-MMP) and does not cross-react with the
other MMP family members (MMP-1, MMP-2, MMP-3,
MMP-9, etc). The zymogen for MMP-17 is approx-
imately
Product Information Sheet - H0393
et al., Nat. Biotech., 6, 72-77 (1988).
7. The Merck Index, 11th ed., Entry# 4638 (1989).
8. Jiang, S.-Y. et al., PLoS One, 8(6), e64336 (2013).
IDC,GCY,CS,JWM,MAM 11/17-1
2017 Sigma-Aldrich
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