Search Within
Applications
Content Type
Applied Filters:
Applications:3D cell culture
Content Type:Protocol
Showing 1-20 of 20 results
Cultrex® 96-Well 3D Spheroid BME Cell Invasion Assay Protocol
The 96 Well 3D Spheroid BME Cell Invasion Assay utilizes 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells.
Tumor Spheroid Formation Assay
3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media. Explore over 350 PromoCell products on Sigma.com
Cultrex® 3-D Spheroid Fluorometric Proliferation/Viability Assay Protocol
The 3-D Spheroid Fluorometric Proliferation/Viability Assay offers a high content format for inducing tumor spheroid formation and quantitating cell viability within the spheroids using resazurin fluorescent detection.
Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol
3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.
TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol
TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits
Corning® Spheroid Microplates
Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well ­spheroid microplate format.
Cancer Stem Cell Tumorsphere Formation Protocol
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Fibronectin Coating Protocol
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs
TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol
TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.
TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol
A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available
Cultrex® 3-D Culture Cell Harvesting Kit Protocol
Cultrex™ 3-D Culture Cell Harvesting Kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3-D Culture Matrix™ BME or Laminin I for subsequent biochemical analysis.
In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Cultrex® 3-D Culture Matrix™ Reduced Growth Factor Basement Membrane Extract, PathClear® Protocol
Cultrex® 3-D Culture Matrix™ RGF BME is the first Basement Membrane Extract produced and qualified specifically for use in 3D culture studies.
Poly-L-Lysine Cell Attachment Protocol
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components.
Laminin Coating Protocol
Laminin coating protocol, products and FAQs at sigmaaldrich.com
Cultrex ® 3-D Culture Matrix™ Laminin I Protocol
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
Cultrex® 3-D Spheroid Colorimetric Proliferation/Viability Assay Protocol
The 3-D Spheroid Colorimetric Proliferation/Viability Assay offers high content format for inducing tumor spheroid formation and quantitating cell viability within the spheroids by absorbance using MTT.
Generating organotypic 3D skin cultures as an in-vitro model of the human epidermis
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.