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Extracellular Matrix Proteins and Tools for Cell Culture Optimization
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models
Sophisticated models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays.
What is the Extracellular Matrix?
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Hydrogel Rigidity
The study of matrix stiffness and its effects on cellular behavior has generated a great deal of interest among cell biologists and tissue engineers. The stiffness of a cell substrate can affect cell motility, phagocytosis, and differentiation.
3D Cell Culture with Corning Microplates
Take a deep dive into the world of 3D cell culture courtesy of Corning and understand why the Corning® spheroid microplate can be an asset in the evolution of 3D cell culture.
Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media
Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.
An ECM Mimetic Library for Engineering Surfaces to Direct Cell Surface Receptor Binding Specificity and Signaling
The extracellular (ECM) microenvironment, defined by biochemical cues and physical cues, is a deciding factor in a wide range of cellular processes including cell adhesion, proliferation, differentiation, and expression of phenotype-specific functions. For this reason, engineering the ECM microenvironment provides...
ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts
Cell culture protocols using Sigma ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Three-Dimensional Printing of Tissue Engineering Scaffolds
We will explore the technological advances that have contributed toward the progress of 3DP of tissue engineering scaffolds, current materials used to create 3DP scaffolds, and the challenges that remain.
3D Cell Culture Tools and Techniques
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Co-culture of Tumor and Stromal Cells in TrueGel3D™ Hydrogels
TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.
Cell-in-a-Box® Live Cell Encapsulation Nanotechnology
Cell-in-a-Box® technology allows cell encapsulation using biocompatible semipermeable cellulose nanoparticles for cellular therapy and 3D cell culture applications.
MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications
Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.
Cyst Formation of MDCK Cells in TrueGel3D™ Hydrogels
TrueGel3D synthetic hydrogel can be used to study cyst formation of MDCK cells.
True Gel3D™ Hydrogel Troubleshooting Guide
TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide
Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
High-Throughput 3D Cell Culture Using TrueGel3D® HTS Hydrogel Plates
The TrueGel3D® HTS Hydrogel Plate is a ready-to-use solution to easily establish 3D cell cultures using fully synthetic hydrogels in a simple and automation-compatible manner.
TrueGel3D™ Hydrogel for 3D Cell Culture
TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
Photocrosslinkable Hydrogels for 3D Cell Culture and Tissue Engineering
Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.
dECM hydrogels 3D culture
Comparison of decellularized extracellular matrix (dECM) hydrogels to Matrigel® matrices for predictive 3D cell culture models
High-Throughput Drug Screening using TrueGel3D® HTS Hydrogel Plates
Compound cytotoxicity screening assays with 3D cell cultures using TrueGel3D® HTS Hydrogel Plates including dose response curves, IC50 determination, and spheroid killing assay data.
5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature
Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.
HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications
Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.
Tissue-Specific Decellularized Extracellular Matrix (dECM) Hydrogels for Enhanced 3D Cell Cultures
To isolate the native extracellular matrix of a tissue from its inhabiting cells, a decellularization process is employed.
Create a 3D kidney nephrotoxicity model on the AIM Biotech 3D cell culture chip
AIM Biotech’s easy-to-use modular platform for conducting 3D cell culture into your research. The AIM Biotech organ-on-a-chip enables you to run simple assays or create more complex microphysiological systems. The versatile platform is suitable for a wide variety of cell...
Webinar: Cell Culture in 3D Systems; Moving from a 2D to 3D Environment.
Cell culture in 3D systems: moving from 2D to 3D cell culture? It’s now well accepted by the scientific community that the 3D cell culture condition better recapitulate the in vivo environment and behaviours of cells.
Fibronectin
Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.
Attachment Factors for 3-Dimensional Cell Culture
Attachment Factors for 3-Dimensional Cell Culture
Nanofiber Scaffold Technology Validated in a Historic Human Implant
Technical Article on Nanofiber Scaffold Technology Validated in a Historic Human Implant.
Three-Dimensional Bioprinting for Tissue and Disease Modeling
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
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