Search Within
Applications
Content Type
Applied Filters:
Applications:Cloning and Expression
Content Type:Protocol
Showing 1-29 of 29 results
x-tracta Gel Extraction Tool
A quick, clean and easy to use alternative to using a scalpel or razor blade for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis.
Universal Transfection Reagent Protocol
Our Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. This is a fast and easy protocol is compatible...
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Dephosphorylation Procedures for DNA and Proteins
Dephosphorylation of DNA using Calf Intestinal Alkaline Phosphatase, Shrimp Alkaline Phospha, Bovine Intestinal Alkaline Phosphatase
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA...
Competent Cell Protocols
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
T4 DNA Ligase (Rapid) Protocol
T4 DNA Ligase for joining blunt ends of DNA and RNA.
Preparing Cells for Electroporation
Preparing Cells for Electroporation
Ligation of Insert to pGEX DNA
This page describes ligation of insert to pGEX DNA using Cytiva products.
pGEX Vectors
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Selecting Correctly Expressing Recombinants
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
X-tremeGENE HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Simplicon™ RNA Transfection and Electroporation Protocols
Simplicon™ RNA Transfection and Electroporation Protocols
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Co-transfection of Plasmid DNA
Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
DNA Ligation Protocol
The cloning process requires the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology is essential for many basic experiments in biotechnology.
QuickLink™ DNA Ligation Kit
T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules
Escort™ III Transfection Reagent Protocol
The product bulletin providin detailed use protocol for easy DNA transfection.
Blue-White Select™ Screening Reagent
Blue/white color selection is a routine technique employed by molecular biologists. This technique simplifies the differentiation between colonies/plaques that contain a cloning vector without an insert and those that contain a vector harboring an insert of interest.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
OverExpress™ Electrocompetent Cells
OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.
OverExpress™ Chemically Competent Cells
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024