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Showing 1-26 of 26 results
Detection of GST-tagged Proteins with Western Blot
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
Northern and Southern Blot Protocols & Introduction
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie® stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol.
Brilliant Blue G – Colloidal Concentrate Protocol
Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.
How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System
Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Running a Multiphor II Protocol
This page shows protocols for running 7–24-cm Immobiline DryStrip gels on the Multiphor II Electrophoresis System.
Detection of GST-tagged Proteins with SDS-PAGE
This page describes how to monitor GST-tagged protein levels during expression and purification with SDS-PAGE using Cytiva products
Using IPGbox for Rehydration
This page shows how to use IPGbox for rehydration of Immobiline® DryStrip from Cytiva.
Using DeStreak™ Rehydration Solution
This page shows how to use DeStreak Rehydration Solution.
Sample Application by Cup Loading
This protocol shows how to apply samples by cup loading for isoelectric focusing.
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
Setting Up and Running TruPAGE™ Precast Gels
TruPAGE™ precast gels offer high rigidity for tear resistance and improved protein resolution so researchers can achieve quality separation the first time around.
Precast Agarose Gels for RNA Electrophoresis
Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
Introduction to SDS-PAGE - Separation of Proteins Based on Size
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Using the Strip Holder for Gel Rehydration
This page shows how to use the IPGphor fixed-length Strip Holders from Cytiva for gel rehydration in 2-D electrophoresis.
Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.
Two-Dimensional Separation of Protein Samples
This page describes two-dimensional separation of protein samples with Cytiva products.
Preparing the Manifold
This page shows how to prepare the Manifold tray, which is part of the Ettan™ IPGphor 3 unit.
Inserting Gels into Ettan™ DALT Electrophoresis Units
This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.
Auto2D® Automated 2-D Gel Electrophoresis Device Protocol
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits