Mammalian cell culture
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Media and Supplements in Cell Culture
An overview of basic cell culture media and supplements including fetal bovine serum, basal media, salts, antibiotics, proteins and amino-acids. Free ECACC handbook download.
Counting of Cells
Trypan blue method of cell counting is inexpensive and readily available method. The method is based on principle that living cells exclude trypan blue dye and appear bright, while dead cells take up dye and appear blue. Watch video to
Nutrient Mixture F-10 Ham Formulation
Nutrient Mixture F-10 Ham Formulation
Growth Factors and Cytokines: Classification and Nomenclature
Growth Factors and Cytokines: Classification and Nomenclature
Inhibition of Cell Wall Biosynthesis by Antibiotics
Inhibition of Cell Wall Biosynthesis by Antibiotics
Corning® High Content Screening Microplates – Frequently Asked Questions
A frequently asked questions guide for users of the Corning® high content screening microplates.
Good Cell Banking Practices
Overview of good cell banking practices for cell line cryopreservation purposes.
Cell Analysis Best Practices
How do you assess the state of your cell culture? We’ve got some best practices to share.
Cell Types & Culture Characteristics
An overview of how to classify various cell types (immortalized, primary or stem cells) based on cellular morphology characteristics such as epithelial, endothelial, neuronal or fibroblast. Cell growth characteristics also discussed. Free ECACC handbook download.
Serum for Cell and Tissue Culture FAQs
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Hybridoma Fusion Partners Cell Lines
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
Glycosaminoglycan Sulfation and Signaling
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
3D Neurosphere Culture Media
3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.
Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it
The Microbiological Safety Cabinet and Its Use
Working with cell cultures generates aerosols, posing threat to scientists and co-workers. To protect against aerosols microbiological safety cabinets are used. Watch video how to use microbiological safety cabinets.
Poor Cell Growth Troubleshooting
Ensuring adequate cell growth is a critical part of collecting accurate data with cell cultures. Cells can be cultured in suspension, or as a monolayer that attaches to cultureware, such as a flask, dish or multiwell plate.
Cell Death Troubleshooting in Cell Culture
Here we discuss causes of cell death, and how to assess and prevent these causes.
Biotin in Cell Culture
Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Lipoic (Thioctic) Acid, in Cell Culture
How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Calcium in Cell Culture
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Vitamin E, Tocopherol, in Cell Culture
Importance and uses of vitamin E (tocopherol) in serum-free eukaryotic, including hybridoma and chinese hamster ovary (CHO), cell cultures.
Glucose in Cell Culture
Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Uric Acid in Cell Culture
Uric acid is not a direct additive to any of the classical cell culture media. However, it is present in animal sera. Consequently, uric acid is typically present in complete serum-supplemented classical cell culture systems.
Thiamine in Cell Culture
How thiamine and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of thiamine.
VEGF Pathway
Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.
Hematopoietic Cytokines
Read article on hematopoietic cytokines and hematopoiesis
Riboflavin in Cell Culture
How riboflavin and othercell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of riboflavin.
Cell Freezing and Banking
Cell Freezing and Banking
Fibroblast Growth Factor Family (FGF)
Fibroblast growth factors in cell culture and various growth factors for your research
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