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Optimized PCR-based Detection of Mycoplasma
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Expand™ Long Template PCR System Protocol Troubleshooting
This polymerase mixture produces a high yield of PCR product from genomic DNA.
Extract-N-Amp™ Tissue PCR Protocol for Formalin-fixed Paraffin Embedded Sections
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
DNA Amplification using Illustra PuReTaq Ready-To-Go PCR Beads
This page shows DNA amplification using illustra PuReTaq ready-to-go PCR beads from Cytiva.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Antibody-Enzyme Mediated Hot Start PCR Protocol
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Amplification of Genomic DNA using REDTaq® DNA Polymerase
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.
Integration of Sigma® TransPlex® with Agilent Microarray
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
SeqPlex DNA Amplification Kit Protocol
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
Long & Accurate PCR with RedAccuTaq®
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
Recombinant Selection
Various DNA manipulation techniques from minipreps to gel electrophoresis are discussed for molecular biology experiments.
Successful PCR Amplification with PCRboost™
DNA sample handling: Withstand harsh conditions for extraction, including formalin fixation, to maintain integrity.
Hot Start dNTP Protocol
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
Digital PCR
Digital PCR enables absolute quantification and detection of minority sequences against similar majority sequences, such as somatic mutations.
Saliva DNA Extraction & WGA Amplification
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Rapid Genotyping of Mouse Tissue with Extract-N-Amp Kit
Genotyping mouse tail samples takes 1.5 hours with SYBR Green Extract-N-Amp™ Tissue PCR Kit, cutting time from days, crucial for timely experiments.
Whole Genome Amplification: Blood Card Protocol
Efficient extraction of genomic DNA from blood cards for whole genome amplification and analysis.
Kapa Biosystems Taq DNA Polymerase and PCR Kits
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
Installation & Use of Milli-Q® SQ 2Series Water Purification Systems
View instructional videos and download User Manuals for Milli-Q® SQ 2Series lab water systems. You’ll be up and running in no time. Buy online systems, accessories, cartridges and services.
Annealing Oligonucleotides Protocol
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
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