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Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
Ion-Suppression & Phospholipid Contamination
Sigma-Aldrich.com presents an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Ion Exchange Methodology
Ion Exchange Methodology in Sold Phase Extraction, Counter Ion Selectivity & Ion Exchange and the basic steps.
Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge
Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.
HybridSPE®-Phospholipid Technology
Innovative solid phase extraction (SPE) technology from Supelco, utilizes phospholipid retention mechanism based on highly selective interaction between proprietary zirconia ions (bonded to the stationary phase) and the phosphate moiety consistent with phospholipids and proteins.
Steroid Hormone Analysis in Serum using Supel™ Swift HLB DPX Tips
This application note describes an accurate and sensitive, Supel™ Swift HLB DPX Tips based analysis of a total of 9 steroids (cortisone, cortisol, 11-deoxycortisol, androstenedione, testosterone, dehydroepiandrosterone, 5α-dihydrotestosterone, 17α-hydroxyprogesterone, and progesterone) in blood serum samples using two different standards (UTAK
Complete Workflow for the Analysis of California List of Pesticides in Cannabis by LC-MS/MS and GC-MS/MS
To show product safety, it is mandatory to test cannabis flowers for the presence of pesticides. In this article, we show a complete workflow for sample preparation and analysis of cannabis for the pesticides registered in California, by LC-MS/MS and
Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
QuEChERS Cleanup and Analysis of Veterinary Drugs in Milk by LC/MS/MS
Use QuEChERS methods to analyze samples of milk for veterinary drugs.
LC-MS Analysis of Serum for a Wide Analyte Range Using a Novel HLB SPE Phase
Analysis of 20 pharmaceutical compounds in calf serum using Supel™ Swift HLB SPE cartridge for cleanup and LC-MS determination and another commercially available HLB cartridge for comparison.
A Comparison of Solid Phase Extraction (SPE) Products Used to Purify Peanut Paste Extract for Aflatoxin Analysis
Supel Tox AflaZea SPE produced exceptional analyte recoveries with good reproducibility for all aflatoxins while requiring less than half the sample volume required by competing products.HPLC analysis of all spiked samples after SPE cleanup showed that all compared SPE cartridges
Ion-Exchange SPE Methodology
Normal-Phase Methodology in Solid Phase Extraction Microbiology. In order g003572 for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment.
LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Solid Phase Extraction (SPE) Overview
Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin).
Supel™-Select Polymeric SPE
Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.
Solid Phase Extraction: Reversed-Phase Methodology
Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.
Impact of Mobile Phase Additives on LC-MS Sensitivity, Demonstrated using Spice Cannabinoids
This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.
The Effect of Container Materials on Production of Light Induced Off-Flavors in Milk – A Study Using SPME-GC-MS
Various plastics used as barriers to the production of off-flavors in light exposed milk were examined using HS-SPME with a new Carboxen®/PDMS coated Nitinol fiber followed by GC-MS analysis allowing detection of low MW analytes at levels <1 ng/mL.
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation
Vitamin D deficiency has become a topic of interest in recent publications. Vitamin D regulates the absorption of calcium within the digestive track and is important to promote proper bone growth and prevent osteoprosis in older adults.
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
Supel™ Swift HLB SPE Cartridges ‒ Solid Phase Extraction (SPE) Made Easy
Supel™ Swift HLB SPE is a polymeric sorbent with lipophilic and hydrophilic functional groups used for solid phase extraction of a wide range of analytes with different polarities. It saves time, offers faster flow rates, and minimum signal enhancement/suppression.
Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis
Analyte recovery values from spiked cannabis extracts ranged from 102 to 127% with RSD below 12% for 3 replicates. Excellent recovery values were even observed for Aflatoxins B2 and G2 which were spiked at a low level of 6.1 ppb.
Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
A simplified LC-MS/MS method to determine glyphosate and related compounds using a Supel™ Carbon LC U/HPLC column for stability under higher pH conditions and sufficient retention for the polar analytes in the presence of methanol as an extraction solvent.
Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC
Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.
Analysis of Drugs of Abuse in Urine After Cleanup with HLB Solid Phase Extraction 96-well Plates
This article validates the ability to perform cleanup of urine samples using HLB solid-phase extraction (SPE) for the analysis of opioids via tandem mass spectrometry (LC-MS/MS) after cleanup with the Supel™ Swift HLB 96-well plate.
Replace Classical Liquid-Liquid Extractions with EXtrelut® NT
Simplify your liquid-liquid extractions with our emulsion-free supported-liquid extraction (SLE) single step solution.
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