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The 5-HT4 receptor interacts with adhesion molecule L1 to modulate morphogenic signaling in neurons.

Journal of cell science (2021-02-05)
Simon Bennet Sonnenberg, Jonah Rauer, Christoph Göhr, Nataliya Gorinski, Sophie Kristin Schade, Dalia Abdel Galil, Vladimir Naumenko, André Zeug, Stephan C Bischoff, Evgeni Ponimaskin, Daria Guseva

Morphological remodeling of dendritic spines is critically involved in memory formation and depends on adhesion molecules. Serotonin receptors are also implicated in this remodeling, though the underlying mechanisms remain enigmatic. Here, we uncovered a signaling pathway involving the adhesion molecule L1CAM (L1) and serotonin receptor 5-HT4 (5-HT4R, encoded by HTR4). Using Förster resonance energy transfer (FRET) imaging, we demonstrated a physical interaction between 5-HT4R and L1, and found that 5-HT4R-L1 heterodimerization facilitates mitogen-activated protein kinase activation in a Gs-dependent manner. We also found that 5-HT4R-L1-mediated signaling is involved in G13-dependent modulation of cofilin-1 activity. In hippocampal neurons in vitro, the 5-HT4R-L1 pathway triggers maturation of dendritic spines. Thus, the 5-HT4R-L1 signaling module represents a previously unknown molecular pathway regulating synaptic remodeling.

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Triton X-100, laboratory grade
Protein A-Sepharose® from Staphylococcus aureus, lyophilized powder
IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
Poly-D-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
L-Cysteine, from non-animal source, BioReagent, suitable for cell culture, ≥98%
DL-Cysteine, technical grade
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
Bovine Serum Albumin, cold ethanol fraction, pH 5.2, ≥96%