QuEChERS significantly improves laboratory efficiency and throughput. This procedure requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.
A process in two steps:
Solvent extraction techniques are designed to achieve maximum yield of analytes from the base matrix.
Solvent selection is important to minimize co-extracting compounds.
Analytes are extracted from the matrix with acetonitrile and salts/buffers.
Sample cleanup is necessary to reduce interferences.
Interferences can damage analytical instrumentation and complicate analyte identification and quantification.
The use of PSA, C18, GCB (Graphitized Carbon Black) or Z-Sep sorbents allow removal of sugars, lipids, sterols, organic acids, proteins, carotenoids, chlorophyll and other pigments prior to a GC-MS/MS or LC-MS/MS analysis.
Transfer sample to 50mL tube
Add ACN and internal standard and shake for 1 min
Extraction: Add extraction salts and centrifuge (55227-U for EN15662:2008 and 55234-U for 2007.01 AOAC)
Cleanup: Transfer supernatant to cleanup tube
Shake and centrifuge
a) LC-MS/MS Analysis: Dilute extract with mobile phase
b) GC-MS analysis: Analyze directly or after solvent exchange
QuEChERS Selection Guide
Products for the Extraction Step
Products for Cleanup (QuEChERS) Step
Method – EN15662:2008
Method – AOAC 2007.01
Specialty Products for Method EN15662 or AOAC 2007.01 – Alternatives