HomeQuEChERS Sample Preparation MethodQuEChERS Method for Pesticide Residue Analysis

QuEChERS Method for Pesticide Residue Analysis

QuEChERS significantly improves laboratory efficiency and throughput. This procedure requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

QuEChERS Method for Pesticide Residue Analysis

A process in two steps:

1. Extraction

  • Solvent extraction techniques are designed to achieve maximum yield of analytes from the base matrix.
  • Solvent selection is important to minimize co-extracting compounds.
  • Analytes are extracted from the matrix with acetonitrile and salts/buffers.

2. Cleanup

  • Sample cleanup is necessary to reduce interferences.
  • Interferences can damage analytical instrumentation and complicate analyte identification and quantification.
  • The use of PSA, C18, GCB (Graphitized Carbon Black) or Z-Sep sorbents allow removal of sugars, lipids, sterols, organic acids, proteins, carotenoids, chlorophyll and other pigments prior to a GC-MS/MS or LC-MS/MS analysis.
  1. Homogenize sample
  2. Transfer sample to 50mL tube
  3. Add ACN and internal standard and shake for 1 min
  4. Extraction: Add extraction salts and centrifuge (55227-U for EN15662:2008 and 55234-U for 2007.01 AOAC)
  5. Cleanup: Transfer supernatant to cleanup tube
  6. Shake and centrifuge
    a) LC-MS/MS Analysis: Dilute extract with mobile phase
    b) GC-MS analysis: Analyze directly or after solvent exchange

QuEChERS Selection Guide

Products for the Extraction Step

Products for Cleanup (QuEChERS) Step

Method – EN15662:2008

Method – AOAC 2007.01

Specialty Products for Method EN15662 or AOAC 2007.01 – Alternatives

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