Optimal conditions must be determined for each cell line and application.
- Add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine.
- Aseptically coat culture surface with 1.0 mL/25 cm2 (only). Rock gently to ensure even coating of the culture surface.
- After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
- Allow to dry at least 2 hours before introducing cells and medium.
*NOTE: Step 1 is not necessary for poly-lysine solutions (P4707 and P4832).