ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie® stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol. No prior fixing step is necessary. For convenience, ReadyBlue™ gel stain can be stored at room temperature. For additional protein gel staining products and protein staining gel needs, please visit our Protein Electrophoresis Gel Stains resource page.
Figure 1.12% mPAGE® Bis-Tris gel loaded with 2-fold serial dilutions of BSA and run with MOPS buffer at 200V for 30 minutes. Gel was imaged with white light and no destaining at 5, 15, 30, and 60 minutes. Gel was also imaged after destaining for 60 minutes with white light and with a LI-COR Odyssey® in the 800 channel for fluorescence.
Adding a brief microwave step can decrease staining time and allow for a quick evaluation of staining efficacy. For maximum sensitivity, use the standard protocol.
NOTE: A minimum one-hour full stain is recommended before storing the gel in water.
For additional information and resources in support of your mass spectrometry workflow needs, please visit our Protein Mass Spectrometry resource hub.
ReadyBlue™ Protein Gel Stain features a shelf-life of up to 18 months from the date of manufacture when stored at 2–8 °C or room temperature and protected from light. Stain may be reused up to three times, however there may be a reduction in sensitivity with each subsequent use because of loss of dye. If the stain is reused, then it is the responsibility of the user to confirm proper storage conditions and verify performance.