These products are available in the US, Canada, and select European countries.
PromoCell® endothelial cells are isolated from large blood vessels and microvascular tissues. Shortly after isolation, Human Endothelial Cells are cryopreserved at passage 1 or passage 2 using proprietary, serum-free freezing medium, Cryo-SFM. Each cryovial contains more than 500,000 viable cells after thawing. Human Endothelial Cells from large vessels are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days.
Four different Endothelial Growth Media formulations have been optimized for culture of cells from distinct vascular sources, and with or without bovine hypothalamic extract (ECGS).
All endothelial media are available in two formats: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (set of individual vials with pre-measured supplements) to provide the user with complete control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium generates the complete medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPacks are all also available for individual purchase.
For researchers working with Human Umbilical Vein Endothelial Cells, HUVEC isolated in standard Endothelial Cell Growth Medium or in Endothelial Cell Growth Medium 2 are available to suit specific applications. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Additionally, HUVEC are available which are pre-screened for VEGF (vascular endothelial growth factor) response.
All endothelial media contain the growth factors and supplements necessary for the optimal growth of human endothelial cells (see table below). They do not contain antibiotics or antimycotics and are formulated for use in an incubator with 5% atmospheric CO2
Note: Light flocculation may be seen upon thawing the supplements containing ECGS/heparin. This does not affect the activity. Optional: centrifuge and remove the precipitate under sterile conditions.
Note: The SupplementMix and SupplementPack are delivered thawed and can be frozen after arrival without loss of activity.
Rigid quality control tests are performed for each lot of human endothelial cells.
All lots of Endothelial Cell Growth Media are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of endothelial cell growth media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Endothelial Cells and Growth Medium
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions, please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.