These products are available in the US, Canada, and select European countries.
Epidermal melanocytes, located in the stratum basale with extensions to the suprabasal layers, make up 5-10% of the cells in the epidermis. Epidermal melanocytes produce the protein melanin, which is responsible for pigmentation of the skin, hair and eyes, in addition to a UV-shielding effect on adjacent cells.
PromoCell Normal Human Epidermal Melanocytes are generated from donors with varied skin pigmentation (light, moderate, and dark). Detailed pigmentation information is available upon request. The isolated cells originate from juvenile foreskin or adult skin from the face, breast, abdomen, thighs, and other sites. The media used is either the serum-free, Melanocyte Growth Medium with PMA (Phorbol Myristate Acetate), or the unique serum-free and PMA-free Melanocyte Growth Medium M2. All Normal Human Epidermal Melanocytes are cryopreserved at passage 2 (P2) shortly after isolation, using a proprietary, serum-free freezing medium, Cryo-SFM. Each thawed cryovial contains at least 500,000 viable cells. Human Melanocytes are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days.
Melanocyte Growth Media was developed for the culture of primary human melanocytes and is available in two distinct formulations:
The Melanocyte Growth Medium containing PMA is available in two formats: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option includes a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit includes a 500 mL bottle of Basal Medium and the SupplementPack, which is a set of individual vials with pre-measured supplements that affords the user full control over the complete media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium makes the complete Melanocyte Growth Medium. The Melanocyte Basal Medium (with or without phenol red), SupplementMix, and SupplementPack can also be purchased separately.
The Melanocyte Growth Medium M2 (PMA-free) is only available as Medium (ready-to-use), which includes a 500 mL bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium generates the complete Melanocyte Growth Medium M2. The Melanocyte Basal Medium M2 (with or without phenol red) and the SupplementMix can also be purchased separately.
The Melanocyte Growth Media includes all of the necessary components for the optimal growth of human melanocytes. Melanocyte Growth Media does not contain antibiotics or antimycotics and is formulated for use in an incubator with 5% atmospheric CO2.
The following rigid quality control tests are performed for each lot of Human Epidermal Melanocytes:
All lots of Melanocyte Growth Media are subjected to comprehensive quality control testing. These tests check for growth-promoting activity, adherence rate, and typical cell morphology using cultured primary human melanocytes. Approved in-house lots of Melanocyte Growth Media are used as a reference. Additionally, microbial testing ensures that all lots of Epidermal Melanocyte Growth Media are free of microbial contaminants (fungi, bacteria, mycoplasma).
Human Melanocytes and Growth Media are intended for in vitro use only and not for diagnostic or therapeutic procedures. For safety information please see appropriate MSDS.
Note: PromoCell® media have low or zero serum content and are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead, we recommend using the DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.