These products are available in the US, Canada, and select European countries.
Mononuclear cells (MNC) represent the enriched lymphocyte and monocyte fraction of whole blood. They are isolated from umbilical cord blood and adult peripheral blood of healthy donors. Mononuclear cells from cord blood contain a relatively high percentage of primitive progenitor cells, while mononuclear cells from adult peripheral blood (hMNC-PB) contain a high proportion of mature immune cells.
Mononuclear Cells are produced from freshly collected whole blood, which is carefully separated by low-density gradient centrifugation, effectively removing the granulocytes. To avoid general cell damage, red blood cells are gently depleted by a proprietary protocol rather than using traditional osmotic lysis techniques. The resulting ultrapure Mononuclear Cells do not clump when thawed, and they exhibit superior viability and unaltered biological function. Immediately after isolation, the freshly prepared Mononuclear Cells are cryopreserved using proprietary serum-free freezing medium, Cryo-SFM. Each cryovial contains at least 25 million viable cells after thawing.
The Mononuclear Cell Medium is a highly nutritive short-term maintenance medium, which provides optimal cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood, and other sources such as bone marrow. It is validated for maintenance of Mononuclear Cells for up to 48 hours before proceeding with your experiments. The Mononuclear Cell Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Combining the SupplementMix with the Basal Medium constitutes the complete Medium.
Mononuclear Cell Medium is recommended for
PromoCell® Mononuclear Cell Medium contains all the growth factors and supplements necessary for optimal short-term maintenance of mononuclear cells. The Mononuclear Cell Medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
All lots of Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of Mononuclear Cell Medium is checked for short-term maintenance of unaltered function and viability of mononuclear cells. Approved in-house lots of Mononuclear Cell Media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Rigid quality control tests are performed for each lot of Human Mononuclear Cells.
* Applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays).