These products are available in the US, Canada, and select European countries.
Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. All Smooth Muscle Cells (SMC) possess the same types of filaments, but depending on the tissue of origin, differ significantly in mechanical and physiological properties.
PromoCell® Human Smooth Muscle Cells (SMC) are isolated from normal human tissue of diverse origins including vascular smooth muscle cells from sources like the aorta, the coronary artery, the pulmonary artery, the umbilical artery. SMC isolated from the trachea, the bronchi, and the uterus are also available. Shortly after isolation, all human Smooth Muscle Cells are cryopreserved at passage 2 (P2) using proprietary, serum-free freezing medium, Cryo-SFM (C-29910). Each cryovial contains more than 500,000 viable cells after thawing. Human Smooth Muscle Cells are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days.
The Smooth Muscle Cell Growth Medium 2 was developed for the in vitro cultivation of smooth muscle cells from blood vessels and hollow organs. The medium is optimized for growing primary human cells, but can also be used to culture bovine, porcine, dog, murine, and rat smooth muscle cells. The Smooth Muscle Cell Media is available in two options: “ready-to-use” and “kit”. The “ready-to-use” option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Smooth Muscle Cell Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements), allowing the user full control over the media formulation. Add the SupplementMix or the SupplementPack to Basal Medium to prepare the complete Smooth Muscle Cell Growth Medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack are also available for separate purchase.
Smooth Muscle Cell Growth Medium 2 is recommended for:
The Smooth Muscle Cell Growth Medium 2 contains all the growth factors and supplements necessary for the optimal growth of human smooth muscle cells, including epidermal growth factor, basic fibroblast growth factor, and insulin (see Table 1 for concentrations). Smooth Muscle Cell Growth Medium 2 does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells.
A detailed certificate of analysis (CoA) for each lot is available for download.
All lots of Smooth Muscle Cell Growth Medium are subjected to comprehensive quality control tests using primary human smooth muscle cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested smooth muscle cells. Approved in-house lots of Smooth Muscle Cell Growth Media are used as a reference.
Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for serum-based trypsin neutralization (when subculturing the cells, for example). Instead we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
Intended use for Smooth Muscle Cell Culture system products
PromoCell® Primary Human Smooth Muscle Cells and Media are for in vitro use only and not for diagnostic or therapeutic procedures.