Primary Small Airway Epithelial Cells and Growth Media

These products are available in the US, Canada, and select European countries.

Human Small Airway Epithelial Cell Culture System

The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.

PromoCell® Human Small Airway Epithelial Cells (HSAEpC) are isolated from normal human adult tissues of the distal respiratory tract. Shortly after isolation, all human small airway epithelial cells are cryopreserved at passage 2 (P2) using proprietary serum-free freezing medium, Cryo-SFM. Each cryovial contains at least 500,000 viable cells after thawing. Human small airway epithelial cells are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days.

The Small Airway Epithelial Cell Growth Medium is a serum-free medium developed for the in vitro cultivation of epithelial cells from the distal respiratory system. The medium is optimized for growing primary human cells but can also be used for porcine small airway epithelial cells. The Small Airway Epithelial Cell Growth Medium is available in two options: Medium (ready- to-use) and Medium Kit. The Medium (ready-to-use) consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements) allowing the user full control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium constitutes the complete Small Airway Epithelial Cell Growth Medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack can also be purchased separately.

Small Airway Epithelial Cell Growth Medium Supplementation Details

PromoCell® Small Airway Epithelial Cell Growth Medium contains all of the growth factors and supplements necessary for the optimal growth of human small airway epithelial cells, including epidermal growth factor, insulin, and transferrin (see the table below for media constituent details). Small Airway Epithelial Cell Growth Medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.

Final Small Airway Epithelial Cell Growth Medium supplement concentrations (after addition to the medium)

Preparing Supplemented Media for Small Airway Epithelial Cells

  • Thaw the Small Airway Epithelial Cell Growth Medium SupplementMix or SupplementPack at 15 – 25 °C.
  • Aseptically mix the supplement solutions by carefully pipetting up and down.
  • Transfer the entire contents of each supplement to the Small Airway Epithelial Cell Basal Medium.
  • Close the bottle and swirl gently until a homogeneous mixture is achieved.

Storage and Stability of Small Airway Epithelial Cell Products

  • Upon arrival, immediately store the Small Airway Epithelial Cell Basal Medium at 4 – 8 °C in the dark; do not freeze the Medium. Store the Small Airway Epithelial Cell Growth Medium SupplementMix or SupplementPack at -20 °C.
  • If stored as directed, the products are stable until the expiration date on the label.
  • Once supplements are added to the Small Airway Epithelial Cell Basal Medium, the shelf life of the complete medium is six weeks at 4 – 8 °C. Do not freeze the complete medium.
  • For use, prewarm only an aliquot of the complete Small Airway Epithelial Cell Growth Medium and keep the remaining medium refrigerated at 4 – 8 °C.
  • After delivery, cryopreserved cells must be seeded directly, or immediately stored in liquid nitrogen.
  • Proliferating cells must be processed immediately.

Small Airway Epithelial Cell Culture System Quality Control

Quality Control for Small Airway Epithelial Cells

Rigid quality control tests are performed for each lot of Small Airway Epithelial Cells.

  • Tested for cell morphology, adherence rate, and cell viability.
  • Flow cytometric analyses for cell-type specific marker cytokeratin are performed for each lot.
  • Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics.
  • Tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

A detailed certificate of analysis (CoA) for each lot are available for download.

Small Airway Epithelial Cell Specifications

Small Airway Epithelial Cell Growth Medium Quality Control

All lots of PromoCell Small Airway Epithelial Cell Growth Medium are subjected to comprehensive quality control tests using primary human small airway epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested small airway epithelial cells. Approved in-house lots of Small Airway Epithelial Cell Growth Media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Intended Use for Small Airway Epithelial Cell Culture Products

These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions, please see appropriate SDS.

Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.

Materials
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