Figure 1.Five individual clones from MISSION® shRNA Target Set NM_002467 were co-transfected with a lentivirus packaging plasmid into HEK 293T cells. The resulting lentiviral particles were used to infect the human lung cancer cell line, H1299. 24 hours post-infection, 2.5 mg/mL of puromycin was added to select for infected cells. Infection efficiency was approximately 95%. 72 hours post-selection, cells were harvested. C-MYC message RNA level was detected by real-time PCR. Actin mRNA was used as an internal control.
Figure 2.Protein lysates from the shRNA infected cells were analyzed by western blotting using a c-MYC antibody. C-MYC protein levels corresponds to the mRNA knockdown shown in Figure 1.
Data generously provided by Dr. Steven B. McMahon, The Wistar Institute
For questions about the library, pricing and quotes or other concerns, please email us.
MISSION is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. Label License.