MITOISO2
Mitochondria Isolation Kit
sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells
Synonym(s):
Mitochondrial Extraction Kit, Mitochondrial Purification Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Quality Level
usage
sufficient for 50 applications (2-5 x 107 cells)
technique(s)
ELISA: suitable, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.
Application
Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
- Ewing′s Sarcoma Family of Tumor (ESFT) cells
- blood samples of tuberculosis patients
- human neuroblastoma cells and glioma cells
- myotubes
- HepG2 cells
Features and Benefits
- The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
- The isolated mitochondria will mostly have intact inner and outer membranes.
- Can be used to extract mitochondrial proteins for proteome analysis
Other Notes
- This product is for R&D use only, not for drug, household, or other uses.
- Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
- Use ultrapure water for the preparation of reagents.
- Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.
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Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
signalword
Warning
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MITOISO2-1KT | 04061834066781 |