Merck
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C6079

Sigma-Aldrich

Collagenase + protease inhibitor

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Enzyme Commission number:
NACRES:
NA.54

biological source

bacterial (Clostridium histolyticum)

Quality Level

form

solid

specific activity

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

mol wt

68-130 kDa

shipped in

dry ice

storage temp.

−20°C

Gene Information

Application

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Biochem/physiol Actions

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Other Notes

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

Quality

Also contains clostripain and non-specific neutral protease activities.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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