Merck
All Photos(1)

03-0028-00

Millipore

SMC® Human Interleukin 1 Beta (IL-1β) Immunoassay Kit

eCl@ss:
32161000

species reactivity

human

Quality Level

manufacturer/tradename

SMC®

assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

Interleukin-1 alpha and beta (IL-1α and IL-1β) are pro-inflammatory cytokines involved in immune defense against infection, and are part of the IL-1 superfamily of cytokines. Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. IL-1 is involved in various immune responses with a primary role in inflammation, making it a target for Rheumatoid Arthritis (RA). IL-1α and IL-1β are produced as precursor peptides, which are proteolytically processed and released in response to cell injury, and thus induce apoptosis. IL-1β production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever.
The SMC® IL-1β Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® Immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) gives the SMC® Human IL-1β Immunoassay Kit unparalleled performance inthe quantification of IL-1β in human EDTA plasma.
Used to detect/quantify:IL-1β

Components

IL-1β Coated Beads
Standard Diluent
IL-1β Detection Antibody
Assay Buffer
Human IL-1β Standard
SMC® Human IL-1β Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D

Analysis Note

Low-end CV% Range: 2 - 10%|Low-end CV% Average: 6%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service