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SMC® Human Interleukin 1 Beta (IL-1β) Immunoassay Kit


species reactivity


Quality Level



assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)


ELISA: suitable
single molecule counting: suitable

General description

Interleukin-1 alpha and beta (IL-1α and IL-1β) are pro-inflammatory cytokines involved in immune defense against infection, and are part of the IL-1 superfamily of cytokines. Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. IL-1 is involved in various immune responses with a primary role in inflammation, making it a target for Rheumatoid Arthritis (RA). IL-1α and IL-1β are produced as precursor peptides, which are proteolytically processed and released in response to cell injury, and thus induce apoptosis. IL-1β production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever.
The SMC® IL-1β Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® Immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.


Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) gives the SMC® Human IL-1β Immunoassay Kit unparalleled performance inthe quantification of IL-1β in human EDTA plasma.
Used to detect/quantify:IL-1β


IL-1β Coated Beads
Standard Diluent
IL-1β Detection Antibody
Assay Buffer
Human IL-1β Standard
SMC® Human IL-1β Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D

Analysis Note

Low-end CV% Range: 2 - 10%|Low-end CV% Average: 6%

Legal Information

SMC is a registered trademark of Singulex


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial


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Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids



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