Merck
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03-0137-00

Millipore

SMC® Mouse TNF-α Discovery Immunoassay Kit

Synonym(s):
mouse TNF-a immunoassay kit, mouse TNF-alpha Single Molecule Counting Kit, mouse TNF-alpha immunoassay kit, mouse TNF-alpha ultra-sensitive immunoassay
eCl@ss:
32161000

manufacturer/tradename

SMC®

Quality Level

assay range

sensitivity: 0.49-500 pg/mL
accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

The SMC® Mouse TNF-α Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn′s disease, psoriasis and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Mouse TNF-α Discovery Immunoassay unparalleled performance in the quantification of TNF-α in mouse serum.

Components

Assay Buffer
Mouse TNF-α Coating Antibody
Assay Plate
10X PBS
Blocking Buffer
Standard Diluent
Mouse TNF-α Detection Antibody
Mouse TNF-α Standard
SMC® Mouse TNF-α Immunoassay Kit Instructions
10X Wash Buffer
Buffer C
Elution Buffer B

Analysis Note

Low-end CV% Range: 0-16%|Low-end CV% Average: 6%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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