Merck
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03-0176-00

Millipore

SMC® Human IL-10 High Sensitivity Immunoassay Kit

species reactivity

rat, mouse, feline, canine, nonhuman primates

manufacturer/tradename

SMC®

assay range

sensitivity: 0.10 pg/mL
(Lower Limit of Quantification:)

inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay n=20; Inter-assay n=5)

technique(s)

single molecule counting: suitable

storage temp.

2-8°C

General description

The SMC® Human IFN-b1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Specificity

Species Reactivity
Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
  • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%

Application

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.

This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-10 kit, #03-0056-00:
  • Improved LLOQ--0.10 pg/mL in the new streamlined kit while the legacy kit is 0.39 pg/mL
  • Adjusted standard curve--changed to 0.1 – 100 pg/mL from 0.17 – 400 pg/mL
  • Improved options for sample prep and incubation
  • Lyophilization of the standard provides the kit at one storage condition
  • Removal of a plate transfer step
  • Increased reagent volumes to accommodate automation
  • Validated to run on both SMCxPRO® and Erenna


Research Category:
Inflammation & Immunology

Research Sub-Category:
  • Inflammatory Disease
  • Immune Response
  • Autoimmune disease

Packaging

96 well assay

Components

  • Assay Buffer
  • IL-10 Coated Beads
  • Standard Diluent
  • IL-10 Detection Antibody
  • IL-10 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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