The Sigma-Aldrich Anti-α-Actin Mouse mAb (1A4) with the product number 113200 is the same "clone" as the Sigma-Aldrich Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 with the product number CBL171-I. Clone ASM-1, also known as anti-αsm-1 and clone 1A4. The Anti-Actin smooth muscle Antibody, clone ASM-1/1A4 (product number CBL171-I) shows reactivity in human, mouse, rat, chicken, and bovine species. Accordingly, the Anti-α-Actin Mouse mAb (1A4) with the product number 113200 should cross-react with bovine smooth muscle actin since it is the same clone as in CBL171-I.
113200
Anti-α-Actin Mouse mAb (1A4)
liquid, clone 1A4, Calbiochem®
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
1A4, monoclonal
Species reactivity:
baboon, rabbit, human
Application:
—
Citations:
12
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
antibody product type
primary antibodies
clone
1A4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, rabbit, human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (1:50-1:75)
Immunoblotting
Paraffin Sections (1:50-1:75))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ACTA1(58)
General description
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
- Antibody Target Gene Symbol: ACTA2
- Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
- Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
- Hu Entrez ID: 59 (Related Antibodies: 113200 )
- Rat Entrez ID: 81633
This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
Immunogen
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Application
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Analysis Note
Negative Control
Fibroblasts, striated muscle, or myocardium
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Other Notes
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description liquid, clone 1A4, Calbiochem® | description clone BM-75.2, ascites fluid | description IgG fraction of antiserum, buffered aqueous solution | description liquid, clone 1A4, Calbiochem® |
| species reactivity baboon, rabbit, human | species reactivity mouse, bovine, human, chicken | species reactivity wide range (animal), invertebrates | species reactivity human |
| Gene Information human ... ACTA1(58) | Gene Information human ... ACTN1(87) | Gene Information human ... ACTA1(58) | Gene Information human ... ARSB(411) |
| clone 1A4, monoclonal | clone BM-75.2, monoclonal | clone polyclonal | clone 1A4, monoclonal |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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Could you provide information on the reactivity of antibody 113200-500UL with bovine cells? The website does not mention reactivity for bovine species, and I would like to use it with bovine cells.
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