126579

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

• Synonym(s): Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• NACRES: NA.25
• UNSPSC Code: 12352202
• eCl@ss: 32160409
• Assay: ≥95% (Albumin, electrophoresis)
• Form: lyophilized powder
• Technique(s): immunofluorescence: suitable
• Impurities: ≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture

Properties

• description: Merck USA index - 14, 8468
• Quality Segment: 100
• assay: ≥95% (Albumin, electrophoresis)
• form: lyophilized powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): immunofluorescence: suitable
• impurities: ≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture
• color: pale green
• solubility: aqueous buffer: 20 mg/mL, water: 20 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Preparation Note

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Material may take up to 24 h to fully dissolve.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable