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126593

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

Synonym(s):

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

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About This Item

CAS Number:
MDL number:
NACRES:
NA.25
UNSPSC Code:
12352202
Assay:
≥98% (agarose gel electrophoresis)
Form:
solid
Impurities:
≤3.0 ash, ≤5.0% moisture
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description

Merck USA index - 14, 8468

Quality Segment

assay

≥98% (agarose gel electrophoresis)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤3.0 ash, ≤5.0% moisture

color

off-white

solubility

0.15 M sodium chloride: 10 mg/mL

cation traces

heavy metals: ≤10 ppm

shipped in

ambient

storage temp.

2-8°C

General description

Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.

Application

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
  • to block tissue sections of placenta in immunohistochemical (IHC) analysis
  • as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
  • as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Biochem/physiol Actions

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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description

-

description

-

description

BSA Fatty Acid-free is designed for use in serological testing, RIA, and hormone response studies. Suitable for use in Molecular Biology applications, such as Northern and Southern blots.

description

-

assay

≥98% (agarose gel electrophoresis)

assay

≥95% (cellulose acetate electrophoresis)

assay

≥98% (SDS-PAGE)

assay

≥96% (SDS-PAGE)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

solid

form

powder

form

solid

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

≤5.0% moisture, ≤3.0 ash

impurities

≤5% moisture, ≤3% ash, ≤0.02% fatty acids

impurities

≤0.02% fatty acids, ≤3.0% Ash, ≤5% moisture

impurities

≤5% Moisture

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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