217594

Anti-CD44 Rat mAb (A020)

liquid, clone A020, Calbiochem^®

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: A020, monoclonal
• Species reactivity: canine, bovine, human, rabbit, porcine, mouse
• Application: —
• Citations: 13

Properties

• biological source: rat
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: A020, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: canine, bovine, human, rabbit, porcine, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA (1:2000); Flow Cytometry (1:1000); Immunoblotting (1:1000); Immunofluorescence (1:200); Immunohistochemistry (1:250); Immunoprecipitation (1:200); Neutralization Studies ; Radioimmunoassay (1:5000))
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... Cd44(281057) ; dog ... Cd44(403939) ; human ... CD44(960) ; mouse ... Cd44(12505) ; pig ... Cd44(100525150)

Description

General description

Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.

Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.

Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.

Immunogen

Human

purified human lymphocyte CD44

Application

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:200)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Neutralization Studies (see comments and application references)

Radioimmunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.

The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable