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Belysa Immunoassay Curve Fitting Software

Synonym(s):

Belysa Software, Curve Fitting Tool

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About This Item

NACRES:
IB.31
UNSPSC Code:
12352200
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description

analysis of data from Luminex®, ELISA and SMCxPRO®

technique(s)

ELISA: suitable (data analysis)

storage temp.

no temp limit

General description

Belysa allows the user to analyse the data within a .CSV output file from Luminex® (RUO Magpix, LX200, FM3D & INTELLIFLEX) multiplex immuoassay readers, the SMCxPRO® and ELISA platforms. Beginning with the application and fitting of different curve models (4PL, 5PL, Linear etc.) the software further allows the user to evaluate thier 96 or 384 well assay for deviations such as replicate %CV or the expected recovery of a standard curve. A user deffined flag system allows the user to easily ID wells that require a closer look while also giving information on the data point such as its postion with regards to the LLOQ or LOD of the assay. When happy with the individual plate a further feature allows for the comparison of multiple standard curves, either within run or between assay lots, using a slope ratio to evaluate for similarity. By allowing for the close evaluation of the single plate and multiple curves Belysa allows you to confirm that your method was reproducable time and again.

Application

This software is used to evaluate the data from a run of 96 or 384 well immunoassay, obtained from a Luminex®, ELISA or SMCxPRO® platform, for method constistency. Software functionality inclues; Plate Map creation, Curve Fitting, %CV, %Ρecovery, result calculation and standard curve comparison.

Research Category:
  • Milliplex®
  • ELISA
  • SMCxPRO®

Packaging

1 Box (containing two USBs)

Legal Information

Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

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Questions

1–4 of 4 Questions  

  1. Is the Belysa software compatible with Windows and MacOS?

    Is the Belysa software compatible with Windows and MacOS?

    1 answer

    1. Belysa can now be run on both Windows 10 and 11, as well as MacOS (beta version). They can also be downloaded from: https://www.sigmaaldrich.com/services/software-and-digital-platforms/belysa-immunoassay-curve-fitting-software/belysa-downloads

      Helpful?

  2. Is it possible for Belysa to perform a 4PL curve fitting with 1/Y weighting, and if so, could a brief overview be provided on the procedure to accomplish this?

    1 answer

    1. The weighting function in the software is not customizable. It provides two weighting functions that are automatically selected based on the curve fitting formula applied. The user manual provides more detail on this topic.

      Weighting

      The weighting function affects how the curve fit parameters are calculated and describes how the variance changes in relation to the concentration. For most curve fit options, the weighting function used is 1/y². For Robust curve fit options, the weighting function used is 1 divided by the median value of the replicate group (or the average if 2 or fewer replicates).

      Helpful?

  3. 1. Why does MDD sometimes display a value when blanks haven't been defined in the plate map? LOD was N/A, but MDD showed values.

    2. Do blank wells contribute to the curve fit equation, or is it only non-zero standard points?

    1 answer

    1. 1. In version 1.2.1, both LoD and MDD are interpolated from the standard curve. If the curve does not have any blanks defined, it is expected that the LoD and MDD would be non-applicable (N/A). It is important to note that the software does not designate a separate "blank" well type. Any standard well with an expected concentration of 0.0 is treated as a blank for the purposes of calculating LoD and MDD.

      2. Yes, the curve fitting process does take into account blank wells. Therefore, the presence or absence of blank wells within the group will affect the curve fitting.

      Helpful?

  4. Is there a way within the Belysa software to apply a dilution factor to every analyte at once? Also, is there a way to copy and paste a dilution factor down a column of samples within the software?

    1 answer

    1. The best and easiest way to use sample dilutions in Belysa is to have them in the input file (.csv). So either enter into Xponent and have the sample dilutions get exported out of Xponent with your raw data or edit your .csv file to add the desired sample dilution to the end of the file where sample dilutions are kept.

      Helpful?

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