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Terrific Broth - Novagen

Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli.

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Synonym(s):
TB media

Quality Level

form

granular

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

10-30°C

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manufacturer/tradename

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manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

application(s)

microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

microbiology

application(s)

food and beverages

storage temp.

10-30°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

100

General description

Terrific Broth (TB) is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures. Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli. The granules ensure rapid and uniform dissolution in water, and prevent clumping of the medium and inhalation of the airborne powder. The composition of the Terrific Broth per liter is 12 g tryptone, 24 g yeast extract, 9.4 g potassium phosphate, dibasic, and 2.2 g potassium phosphate, monobasic. Use 47.6 g of granulated medium per liter of water.

Application

Terrific Broth – Novagen has been used to culture BLR(DE3) bacteria for protein polymer synthesis and production of recombinant antigens for autoantibody assays

Features and Benefits

  • Media preparation is simple and quick
  • ready to use EasyPak media packets: Just add water and sterilize
  • Consistent, high-quality media for E.coli cells

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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