Unfortunately, the original publication does not specify the sex of the mouse from which the microglia were derived. This information is not available.
SCC134
IMG Mouse Microglial Cell Line
Mouse
Synonym(s):
Immortalized microglial cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
mouse
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Product Name
IMG Mouse Microglial Cell Line, IMG microglial cells recapitulate key features of microglial cell activation and may be used to study neuroinflammatory responses underlying Alzheimer′s disease.
biological source
mouse
technique(s)
cell based assay: suitable
shipped in
ambient
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source mouse | biological source mouse | biological source human | biological source mouse |
| shipped in ambient | shipped in ambient | shipped in - | shipped in ambient |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level - |
General description
Immortalized MicroGlial Cell Line or IMG is an immortalized microglial cell line derived from adult murine brain infected with the v-raf/v-myc retrovirus. IMG cells express markers that are specific to adult primary microglial cells (F4/80 and CD11b) as assessed by flow cytometry and are responsive to pro- and anti-inflammatory signals (1). They have been shown to phagocytose foreign particles and misfolded proteins such as the amyloid-beta peptide (1). IMG cells are rapidly dividing cells that are typically small and round with few or no processes when cultured in medium containing serum.
Reference:
1. McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH and Wessling-Resnick M (2016). Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J. Neuroinflammation 13: 21
Reference:
1. McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH and Wessling-Resnick M (2016). Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J. Neuroinflammation 13: 21
Microglia are the primary immune cells of the central nervous system (CNS), and account for 10-15% of all cells found within the brain. Microglial cells play an important role in maintaining the health of the CNS by the removal of pathogens, infectious agents and damaged cells through phagocytosis. Microglial cells have also been extensively studied for their harmful roles in a variety of neurodegenerative disease including Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
Application
Research Category
Immune Response
Inflammation & Immunology
Neuroscience
Immune Response
Inflammation & Immunology
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neuroinflammation & Pain
Neurodegenerative Diseases
Neuroinflammation & Pain
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].
Biochem/physiol Actions
Macrophage Cells
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
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Are these microglia from male or female mice?
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What kind of media would be best to use for this cell line?
1 answer-
The recommended medium for this cell line is as follows:
High Glucose DMEM (Cat. No. D6546)
10 % FBS (Cat. No. ES-009-B)
1X L-Glutamine (Cat. No. TMS-002-C)
1X Penicillin-Streptomycin Solution (Cat. No. TMSAB2-C) (optional)Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/145/570/20267563.pdfHelpful?
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