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10109886001

Roche

Trypsin Inhibitor

from soybean

Synonym(s):
trypsin

biological source

soybean

Quality Level

form

lyophilized

mol wt

Mr 20,100

packaging

pkg of 50 mg

manufacturer/tradename

Roche

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

pH

3.8-4.5

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

General description

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Specificity

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Application

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
  • in the buffer for hepatocyte isolation
  • to inhibit trypsin in Corneal epithelial cells
  • to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Biochem/physiol Actions

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Specifications

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Unit Definition

1 U = 270 S & T-units or 90 USP-units

Preparation Note

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Reconstitution

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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