Merck
All Photos(1)

38587

Millipore

RLS Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):
Rapid Lemonade Spoilage Organism Broth, Rapid Lemonade Spoiling Organism Broth, SSL Broth

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

D(+)-Biotin (Vitamin H), 100 μg/L
Fructose, 10 g/L
Glucose, 30 g/L
Malt Extract, 200 g/L
Orange Extract (=Orangenserum 10 °Brix (60 °Brix) 400g/l (60 g/l)), 40 g/L
Saccharose, 30 g/L
Yeast Extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

5.0±0.2 (25 °C)

application(s)

bioburden testing
food and beverages
sample preparation

microbiology

suitability

Acetobacter spp.

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form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

-

composition

D(+)-Biotin (Vitamin H), 100 μg/L , Fructose, 10 g/L , Glucose, 30 g/L , Malt Extract, 200 g/L , Orange Extract (=Orangenserum 10 °Brix (60 °Brix) 400g/l (60 g/l)), 40 g/L , Saccharose, 30 g/L , Yeast Extract, 3.0 g/L

composition

-

composition

brilliant green, 0.015 g/L , disodium hydrogen phosphate, 6.45 g/L , glucose, 5 g/L , ox-bile, 20 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 2 g/L

composition

ammonium chloride, 1 g/L , disodium hydrogen phosphate, 6 g/L , potassium dihydrogen phosphate, 3 g/L , sodium chloride, 0.5 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

final pH

5.0±0.2 (25 °C)

final pH

6.8±0.2 (25 °C)

final pH

7.2±0.2 (20 °C)

final pH

7.4±0.2 (25 °C)

Application

Rapid enrichment medium for the beverage spoiling microorganisms. It can be used for all kind of beverage, base ingredients or swap samples in the soft drink, dairy and winery industry.

Preparation Note

Dissolve 313 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes or sterile filter the solution. For normal beverages mix add 50 ml broth to 200 mL of sample. For base ingredients 10-20 mL of sample are added to 50 mL broth. For filtered samples the rolled membrane is given in a sterile tube or flask with broth. It is recommended to degas carbonated beverages before performing analysis. Incubate under aerobic conditions for 2 days at a temperature of 25-29°C.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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