Merck
All Photos(1)

56156

Millipore

M-17 Broth

suitable for microbiology, NutriSelect® Basic, selective medium for Streptococcus thermophilus

All Photos(1)
NACRES:
NA.85

Quality Level

100

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

ascorbic acid, 0.5 g/L
lactose, 5 g/L
magnesium sulfate, 0.25 g/L
meat extract, 5 g/L
meat peptone (peptic), 2.5 g/L
sodium glycerophosphate, 19 g/L
soya peptone (papainic), 5 g/L
tryptone, 2.5 g/L
yeast extract, 2.5 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

pH

7.0±0.2 (25 °C)

application(s)

food and beverages
microbiology

storage temp.

2-25°C

suitability

nonselective for Enterococcus spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.

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M-17 Broth suitable for microbiology, NutriSelect® Basic, selective medium for Streptococcus thermophilus

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sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

-

form

powder

form

powder (dehydrated (DCM))

form

powder

form

liquid

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

ascorbic acid, 0.5 g/L , lactose, 5 g/L , magnesium sulfate, 0.25 g/L , meat extract, 5 g/L , meat peptone (peptic), 2.5 g/L , sodium glycerophosphate, 19 g/L , soya peptone (papainic), 5 g/L , tryptone, 2.5 g/L , yeast extract, 2.5 g/L

composition

-

composition

ammonium chloride, 1 g/L , disodium hydrogen phosphate, 6 g/L , potassium dihydrogen phosphate, 3 g/L , sodium chloride, 0.5 g/L

composition

-

packaging

pkg of 500 g

packaging

-

packaging

-

packaging

ampule of 2 mL, pack of 50 tests

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

GranuCult®

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

-

General description

M-17 Broth is a selective medium for Streptococcus thermophilus in the common examination of yoghurt. It is superior to other comparable culture media for the cultivation of the fastidious species such as S. cremoris, S. diacetilactis and S. lactis. Mutants which are incapable of metabolizing lactose can also be isolated on these media. Tryptone, meat peptone, soya peptone, yeast extract and meat extract provide carbonaceous, nitrogenous compounds, vitamin B complex and other essential growth factors. Lactose is the fermentable carbohydrate and ascorbic acid is stimulatory for the growth of lactic Streptococci. Magnesium sulphate provides essential ions to the organisms. Sodium glycerophosphate increases the buffering capacity of the medium, this promotes the growth of lactic streptococci and inhibits lactobacilli growth.

Application

M-17 Broth is a selective medium for Streptococcus thermophilus for examination of yogurt and for the enrichment Streptococcus thermophilus in the dairy industry.

Preparation Note

Suspend 42 g of powder in 1 litre of distilled water and let it soak. Heat to boiling and dispense into suitable containers. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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