63011

M9 Broth

suitable for microbiology, NutriSelect^® Basic

Properties

• sterility: non-sterile
• Quality Level: 100
• form: powder
• composition: ammonium chloride, 1 g/L ; disodium hydrogen phosphate, 6 g/L ; potassium dihydrogen phosphate, 3 g/L ; sodium chloride, 0.5 g/L
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): food and beverages; life science and biopharma; microbiology
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

Application

M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987).

Preparation Note

Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves