Merck
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69965

Millipore

Mossel Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):
Buffered Glucose-Brilliant Green Bile Broth, EE Broth, Enterobacteriaceae Enrichment Broth acc. to Mossel
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.015 g/L
disodium hydrogen phosphate, 6.45 g/L
glucose, 5 g/L
ox-bile, 20 g/L
peptone, 10 g/L
potassium dihydrogen phosphate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (20 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective for Citrobacter spp.
selective for Enterococcus spp.
selective for Escherichia coli
selective for Klebsiella spp.
selective for Proteus spp.
selective for Salmonella spp.
selective for Shigella spp.
selective for Yersinia spp.
selective for coliforms
selective for enterobacteriaceae

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Mossel Broth suitable for microbiology, NutriSelect® Basic

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EC Broth

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.015 g/L , disodium hydrogen phosphate, 6.45 g/L , glucose, 5 g/L , ox-bile, 20 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 2 g/L

composition

bile salts No. 3, 1.5 g/L , di-potassium hydrogen phosphate, 4.0 g/L , D(+)-glucose, 2.5 g/L , novobiocin, 0.02 g/L , peptone from casein, 17.0 g/L , peptone from soymeal, 3.0 g/L , sodium chloride, 5.0 g/L

composition

dipotassium hydrogen phosphate, 2 g/L , glucose, 20 g/L , magnesium sulfate heptahydrate, 0.2 g/L , manganous sulfate tetrahydrate, 0.05 g/L , meat extract, 8 g/L , peptone, 10 g/L , sodium acetate trihydrate, 5 g/L , triammonium citrate, 2 g/L , yeast extract, 4 g/L

composition

bile salts, 1.5 g/L , casein digest (pancreatic), 20 g/L , dipotassium phosphate, 4 g/L , lactose, 5 g/L , potassium phosphate, 1.5 g/L , sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

For the selective enrichment of all enterobacteriaceae from food and other material.

Preparation Note

Dissolve 43.5 g in 1 litre distilled water and heat at 100°C in water bath or flowing steam for 30 minutes. Cool quickly. This medium is heat sensitive. DO NOT AUTOCLAVE.

Other Notes

Isolation of Serratia marcescens from clinical samples

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Customers Also Viewed

Slide 1 of 6

1 of 6

G.M. Grasso et al.
Epidem. Inf., 101, 315-315 (1988)
D.A.A. Mossel et al.
The Journal of Applied Bacteriology, 26, 444-444 (1963)

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Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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