E4378

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

≥97.0%

• Synonym(s): EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
• Linear Formula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂
• CAS Number: 67-42-5
• Molecular Weight: 380.35
• Beilstein: 1717370
• EC Number: 200-651-2
• MDL number: MFCD00004291
• PubChem Substance ID: 24278421
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 400
• Assay: ≥97.0%
• form: powder
• loss: ≤1.0% loss on drying
• mp: 241 °C (dec.) (lit.)
• solubility: 1 M NaOH: 1.1 g/10 mL, clear, colorless
• storage temp.: room temp
• SMILES string: OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used as a component of:

• ice-cold cardioplegia solution for transverse aortic constriction in rats
• ice-cold lysis buffer for protein extraction and whole extract cells
• buffer A, B and, C for the extraction of mitochondria from HEK293F cells

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Physical properties

solubility in water increases with pH

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves