X100

Triton^™ X-100

laboratory grade

• Synonym(s): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• PubChem Substance ID: 329830772
• NACRES: NA.21

Properties

• grade: laboratory grade
• Quality Level: 200
• description: non-ionic
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• aggregation number: 100-155
• CMC: 0.2-0.9 mM (20-25°C)
• transition temp: cloud point 65 °C; pour point ~7 °C
• solubility: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• HLB: 13.5
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton^™-X has been used:

• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F
• Flash Point(C): 251 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves