Merck
All Photos(1)

Documents

270733

Sigma-Aldrich

Water

suitable for HPLC

Sign Into View Organizational & Contract Pricing

Synonym(s):
Aqua, Dihydrogen oxide
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
WX0008006121.00577
Sigma-Aldrich

270733

Water

Supelco

WX0008

Water

Supelco

00612

Water

Water for headspace gas chromatography SupraSolv&#174;

1.00577

Water

form

liquid

form

liquid

form

-

form

liquid

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

ion chromatography: suitable

technique(s)

GC-HS: suitable

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

vapor pressure

3 mmHg

vapor pressure

23 hPa ( 20 °C)

vapor pressure

-

vapor pressure

23 hPa ( 20 °C)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.

Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Supelco

Supelco

95283

Water

Supelco

Supelco

14211

Water

Acetonitrile HPLC Plus, &#8805;99.9%

Sigma-Aldrich

34998

Acetonitrile

Sigma-Aldrich

Sigma-Aldrich

320072

Water

Optimization and validation of HPLC-UV-DAD and HPLC-APCI-MS methodologies for the determination of selected PAHs in water samples
Titato, Guilherme T M et al.
Journal of Chromatographic Science, 44, 35-40 (2006)
Milk ultrafiltrate analysis by ion chromatography and calcium activity for SMUF preparation for different scientific purposes and prediction of its supersaturation
Joseph D et al.
International dairy journal, 68, 60-69 (2017)
Identification of the cysteine protease legumain as a potential chronic hypoxia-specific multiple myeloma target gene
Ada-Sophia C et al.
Cells, 11, 292-292 (2022)
Rapid confirmatory assay for the simultaneous detection of ronidazole, metronidazole and dimetridazole in eggs using liquid chromatography-tandem mass spectrometry
Els D et al.
Analyst, 125, 1533-1535 (2000)
S Bekesova et al.
Journal of proteomics, 75(7), 2216-2224 (2012-02-14)
N-glycosylation of proteins provides a rich source of information on liver disease progression because majority of serum glycoproteins, with the exception of immunoglobulins, are secreted by the liver. In this report, we present results of an optimized workflow for MALDI-TOF

Articles

Optimized separation of Oligo Standard 6, an internally created HPLC-UV system suitability mix, on Chromolith® RP-18e columns with an evaluation of the effect of flow rates up to 3 mL/min and ion-pairing reagent, TEAA, on the separation.

Endocrine disruptors such as BPA can interfere with embryological development. This study shows ultrapure water from a Milli-Q® lab water system to be free of detectable BPA and thus suitable for embryology research.

This application note describes a step-by-step LC-UV-MS workflow for teriparatide peptide mapping, that includes enzymatic digestion, separation conditions, and their high resolution QTOF mass spectrometer based identification.

A complete SEC-UV workflow for the characterization of mAb monomers, aggregates, and fragments using Zenix® and Zenix®-C SEC columns, including system suitability testing and forced pH and temperature stress studies.

Protocols

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service