51411C

Minimum Essential Medium

with Earle′s Balanced Salts, with 2.0 mM L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Minimum Essential Medium Eagle, MEM
• NACRES: NA.75

Properties

• description: for research or for further manufacturing use
• Quality Level: 500
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• components: Earle’s salts (5% CO₂): yes; phenol red: 10.62 mg/L; NaHCO₃: 2200 mg/L; L-glutamine: 290 mg/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro . MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle′s Balanced Salts, which make them suitable for use in atmospheres charged with CO₂.

Packaging

1000 mL

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable