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51412C

SAFC

Minimum Essential Medium

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Minimum Essential Medium Eagle, MEM
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

components

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

storage temp.

2-8°C

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51411C51415C56419C
SAFC

SAFC

51412C

Minimum Essential Medium

SAFC

SAFC

51411C

Minimum Essential Medium

SAFC

SAFC

51415C

Minimum Essential Medium

SAFC

SAFC

56419C

Minimum Essential Medium

form

liquid

form

liquid

form

liquid

form

dry powder

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

components

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

components

Earle’s salts (5% CO2): yes
HEPES: 25 mM
L-glutamine: no

components

Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: 292 mg/L
phenol red: 11 mg/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

500

General description

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.

Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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