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Sigma-Aldrich

Sodium hydroxide solution

BioUltra, for molecular biology, 10 M in H2O

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Linear Formula:
NaOH
CAS Number:
Molecular Weight:
40.00
MDL number:
PubChem Substance ID:
NACRES:
NA.26

grade

for molecular biology

Quality Level

vapor pressure

3 mmHg ( 37 °C)

product line

BioUltra

form

liquid

concentration

10 M in H2O
9500-10500 mmol/L (titration, acidimetric)

technique(s)

ELISA: suitable
immunofluorescence: suitable
western blot: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

14

bp

105-140 °C

mp

-12 - -10 °C (10 - 50 °F)

solubility

water: soluble

density

1.320-1.340 g/cm3

cation traces

Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤100 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: ≤0.08
λ: 280 nm Amax: ≤0.05

SMILES string

[OH-].[Na+]

suitability

suitable for Western blot
suitable for molecular biology

application(s)

agriculture
life science and biopharma

InChI

1S/Na.H2O/h;1H2/q+1;/p-1

InChI key

HEMHJVSKTPXQMS-UHFFFAOYSA-M

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General description

Sodium hydroxide (NaOH) is an inorganic, white solid compound, which is found as pellets, flakes, and granules. This highly caustic metallic base and alkali can readily absorb moisture and carbon dioxide in the air. NaOH is deliquescent and soluble in ethanol, water, and methanol.

Application

Sodium hydroxide solution has been used:
  • to prepare reagent A (0.1 M NaOH) for the pH inactivation of endogenous L-lactate by L-lactate dehydrogenase (L-LDH)
  • as a component of fresh alkaline melting solution for the denaturation of the antisense strand
  • to prepare Type-I collagen hydrogels for microfluidic device fabrication

Biochem/physiol Actions

Sodium hydroxide (NaOH) is used in various industries, including textiles, drinking water, soaps, and detergents, pulp and paper manufacturing, and as a drain cleaner.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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