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95284

Sigma-Aldrich

Water

for molecular biology, suitable for FISH

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Synonym(s):
H2O
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

grade

for molecular biology

Quality Level

sterility

sterile-filtered

form

liquid

quality

sterile filtered

shelf life

limited shelf life, expiry date on the label

technique(s)

FISH: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.34 (lit.)

pH

5-7

conductivity

0.0-5 μS/cm at 25 °C

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

cation traces

Ca: <10 ppb
Mg: <10 ppb

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

  • for cloning and plasmid construction.
  • for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.
  • in RNA isolation.
  • fluorescence in situ hybridization (FISH).
  • to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA.
  • for DNA quantification using quantitative polymerase chain reaction (qPCR).

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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