MREH01

MISSION^® Target ID Library

• NACRES: NA.51

Properties

• packaging: pkg of 100 μL (library); pkg of 200 μL (primer 1); pkg of 200 μL (primer 2)
• Quality Level: 200
• concentration: 1 ng/μL (library); 25 μM (primer 1); 25 μM (primer 2)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.

The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.

The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing

Download deep sequencing data of target ID library.

Packaging

1 set

Components

Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable